Page 41 - CatalogNEP-LS

THEORY(45 Hours)

UNIT I: (15 Hours)

Chromosome Organization:Structure of chromosomes, DNA packaging and Metaphase
chromosomes, centromere, kinetochore, telomere and its importance, Heterochromatin and
euchromatin, Chromosome banding, Polytene and lampbgrush chromosomes.Sex chromosomes,
sex determination and dosage compensation in Drosophila and human.Structure and functions of
cell and its organelles (Nucleus, plasma membrane, mitochondria, Golgi bodies, endoplasmic
reticulumn, ribosomes and lysosomes).

UNIT II: (15 Hours)
Mendelian and non-Mendelian Inheritance:Mendelian inheritance and its modification, Maternal
effect, Epigenetic inheritance,Extranuclear inheritance,Variation chromosome structure and
number. Genetic linkage mapping using molecular markers,cell cycle and its regulation;Protein
sorting in ER, Golgi and targeting of proteins to Mitochondria, secretory and endocytotic
pathway;Cell-Cell signalling: Cell surface receptors, Second messenger system, MAP kinase
pathways, Signalling from plasma membrane to nucleus.
UNIT III: (15 Hours)

Brief description of gene expression:Genetic code, Transcription and translation in prokaryotes
and eukaryotes, Regulation of gene expression, Gene mutation and DNA repair: Consequences of
mutations Occurrence

PRACTICAL(30 Hours)

1. Demonstration of SEM and TEM and study of different micrographs
2. Study of Problems based on Genetics
3. Study of permanent histological slides of testis and ovaries of insects/ mice/rat.
4. Basis of reaction and demonstration of the sites of proteins, nucleic acids, lipids &
carbohydrates in ovaries of insects/rat/mice through slides and photographs.
5. Study of stages of mitosis and meiosis from permanent slides from animal and plant
materials through slides/charts/photographs.

SUGGESTED READINGS:

1. Cell Biology and Molecular Biology by D.Robertis, EDP & DE Robertis E.M.F, 8th ed.,
Saunders & Co. Philadelphia (1995).
2. Cell Biology by C.B. Powar, 3rd ed., Himalaya Pub., Bombay (1984).
3. Advances in Cell and Molecular Biology by Dupraw, E.J., eds. Academic Press, New
York & London (1971).
4. Cell Biology. Pollard, Thomas, D.S.Earnshan, William C. Saunders. USA. (2002).
5. The Cell: A Molecular Approach by Cooper GM & H 5th edition (2009).
6. Molecular Biology of the Cell, 5th edition by Alberts, John Raff. Roberts & Walter
(2008).
7. Cell and Molecular Biology: Concepts and Experiments 5th edition by Gerald Karp. John
Wiley and Saunders. 8. Molecular Cell Biology by Lodish et. al. W.H. Freeman Co. New
York (2008).

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