Page 21 - CatalogNEP-LS

Bot.311 Cell Biology 3+1

LEARNING OBJECTIVES:

 The objective of the present course content is to provide a foundation and
background in cellular and acellular entities of plants
 To provide knowledge regarding cell structure in relation to functions, eukaryotic
genome structure (including nuclear and organellar), and regulatory mechanisms.

LEARNING OUTCOMES:

This course will enable the students to understand:
 About the components of endo-membrane systems and mechanisms governing
intracellular trafficking in plant cells
 About the role of plant cytoskeleton and accessory proteins in major cellular processes of
plants
 About the various components of the eukaryotic nuclear and organellar genome, with
special reference to their regulatory role

THEORY (45 Hours)

UNIT –I: Techniques in Biology (6 Hours)
Principles of microscopy; Light Microscopy; Phase contrast microscopy; Sample Preparation for
light microscopy; Electron microscopy (EM)- Scanning EM and Scanning Transmission EM
(STEM); Sample Preparation for electron microscopy
UNIT 2: Cell as a unit of Life (6 Hours)

The Cell theory; Prokaryotic and eukaryotic cells; Cell size and shape; Eukaryotic Cell
components

UNIT 3: Cell Organelles (15 Hours)
Mitochondria: Structure and function. Chloroplast:Structure and function ER, Golgi body:
Structures and roles. Peroxisomes, Glyoxisomes and vacuole: Structures, composition, functions
in animals and plants Nucleus: Nuclear E n v e l o p e - s t r u c t u r e o f n u c l e a r p o r e
complex; chromatin; molecular organization, DNA packaging in eukaryotes, euchromatin and
heterochromatin, nucleolus and ribosome structure (brief).Chromosome organization:
Morphology, centromere and telomere, chromosome alternation, deletions, duplications,
translocations, inversions. variation in chromosome number, aneuploidy, polyploidy.
UNIT 4: Cell Membrane and Cell Wall (6 Hours)

The functions of membranes; Models of membrane structure; The fluidity of membranes;
Membrane proteins and their functions; Carbohydrates in the membrane; Faces of the membranes;
Selective permeability of the membranes; Cell wall.

UNIT 5: Cell Cycle (6 Hours)
Overview of Cell cycle, Mitosis and Meiosis
UNIT 6: Regulation of gene expression (6 Hours)

Prokaryotes: Lac operon and Tryptophan operon; and in Eukaryotes.

6