Page 104 - CatalogNEP-PS

Reactions: Comparative study of nucleophilicity of acyl derivatives. Reformatsky
Reaction, Perkin condensation.

UNIT- IV: (7 Hours)
Amines and Diazonium Salts: Amines (Aliphatic and Aromatic): (Upto 5 carbons)
Preparation: from alkyl halides, Gabriel‘s Phthalimide synthesis, Hofmann
Bromamide reaction.
Reactions: Hofmann vs. Saytzeff elimination, Carbylamine test, Hinsberg test, with
HNO 2,Schotten – Baumann Reaction. Electrophilic substitution (aniline): nitration, bromination,
sulphonation.
Diazonium salts: Preparation: from aromatic amines.
Reactions: conversion to benzene, phenol, dyes.

UNIT-V: (10 Hours)
Amino Acids, Peptides and Proteins:
Preparation of Amino Acids: Strecker synthesis using Gabriel‘s phthalimide synthesis. Zwitterion,
Isoelectric point and Electrophoresis.
Reactions of Amino acids: ester of –COOH group, acetylation of –NH 2 group, complexation with
2+
Cu ions, ninhydrin test.
Overview of Primary, Secondary, Tertiary and Quaternary Structure of proteins. Determination of
Primary structure of Peptides by degradation Edmann degradation,
N-terminal and C–terminal (thiohydantoin and with carboxypeptidase enzyme).
Synthesis of simple peptides (upto dipeptides) by N-protection (t-butyloxycarbonyl and phthaloyl)
& C-activating groups and Merrifield solid-phase synthesis.

PRACTICAL (30 Hours)

1. Systematic Qualitative Organic Analysis of Organic Compounds possessing monofunctional
groups (-COOH, phenolic, aldehydic, ketonic, amide, nitro, amines) and preparation of one
derivative.
2. Organic Synthesis:
(i) Preparation of Iodoform.
(ii) Preparation of p-bromoacetanilide from acetanilide.
(iii)Preparation of glucosazone.
(iv) Preparation of aspirin.
3. Thin Layer Chromatography
(i) Determination of R f value and purity of organic compounds by use of thin layer
chromatography.
(ii) To analyze the organic compounds by thin layer chromatography.

SUGGUESTED READINGS:

1. Principles of Physical Chemistry by Puri, Sharma and Pathania.
2. Physical Chemistry by S.C. Khetarpal, G.S, Sharma and R.K. Kalia.
3. Moderns Approach to Physical Chemistry by S. Kiran.
4. A text Book of Physical Chemistry by K.K.Sharma and I.K. Sharma
5. Physical Chemistry by P.N.Kapil and S.K.Guglani.
6. Elements of Physical Chemistry by Puri, Sharma and Pathania.
7. Advance Organic Chemistry Reaction Mechanism and Structure by Jerry March.
8. Organic Chemistry by SM Mukherji, SP Singh and RP Kapoor, Vol. I, II & III, New Age
International Publishers
9. Organic Chemistry (Volume II) by I. L Finar

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