Page 121 - CatalogNEP-LS

P. 121

 Understanding of the fundamentals of genetic processes in prokaryotes and eukaryotes.
 Understanding of fundamental genetic processes for all
 organisms, especially through the use of in vivo and in vitro genetic tools.

LEARNING OUTCOMES:

This course will enable the students to:
 Identify and distinguish genetic regulatory mechanisms at different levels
 Plan basic experiments in Microbial genetics
 Describe and summarize experimental work in a correct way.

THEORY (30 Hours)

UNIT-I (6Hours)

Introduction to Microbial genetics; Historically important events and major contributions of
scientists in the field of Microbial genetics; Terminologies employed in microbial genetics and
definitions; Nucleic acid – overview DNA, RNA. Bacterial genome Eukaryotic genome; Viral
genome; Difference between prokaryotic and eukaryotic genome; Mechanisms and role of
prokaryotic genome- an overview
UNIT-II (6Hours)

Structure of DNA – A form, B form, Z form; RNA- tRNA, mRNA, rRNA; Role and Replication
of DNA and RNA; Enzymes involved in Replication and its role. Plasmids, Mitochondrial DNA,
Chloroplast DNA – structure and function.

UNIT-III (8Hours)
Gene structure and expression, principles of operon, gene expression in prokaryote and
eukaryotes, intron and exons, post transcriptional modifications. Regulation of gene expression,
negative expression (lac operon and trp operon), positive regulation (cAMP).

UNIT-IV (10Hours)
Principles of mutation, spontaneous and induced mutation, different types of mutations, selection
principles of mutants. Mutagens and their mode of action, transposable elements and insertion
sequences. DNA damage, DNA repair mechanisms in bacteria. Genetic recombination in bacteria,
mechanisms of recombination, transformation, conjugation, transduction. Recombinant DNA
technology

PRACTICAL (30 Hours)

1. Isolation of genomic DNA from pure cultures of bacteria and fungi.
2. Isolation of bacterial plasmids and Plasmid curring.
3. Qualitative and quantitative assay of DNA by spectrometry and gel-electrophoresis.
4. Inducing mutation by chemicals, physical and biological agents.
5. Transformation and selection of transformants.
6. Amplification of gene of interest by PCR – cloning and expression.