Page 59 - CatalogNEP-LS

 To educate student on concepts of proteins, and enzymes
 To introduce the basic knowledge of cellular metabolism in animals

LEARNING OUTCOMES:

This course will enable the students to understand:
 Students will be taught about proteins, and their biosynthesis,
 The course will also teach about catalytic mechanistic of enzymes, its inhibitors and
regulation
 The course will strengthen the basic ideas about metabolism of carbohydrates, proteins and
lipids

THEORY (45 Hours)

UNIT-I: Elementary Idea of Analytical and Separation Techniques (6Hours)
Ultracentrifugation, electrophoresis and chromatography

UNIT-II: Chemical Nature of Protoplasm (6Hours)
Chemistry of carbohydrates, proteins, lipids and nucleic acids

UNIT-III: Enzymes and Co-Enzymes (15Hours)
Definition, nature, functions; Specificity and classification of enzymes and co-enzymes, enzyme
action; high energy compound: phosphagens, phosphate bonding, formation of ATP, energy
release and oxidation mechanism.
UNIT-IV: Carbohydrate Metabolism (6Hours)

Glycolysis, Kreb‘s Cycle, Pentose phosphate pathway, Gluconeogenesis, Glycogen metabolism,
Review of electron transport chain

UNIT-V: Lipid Metabolism (6Hours)
Oxidation of fatty acids; fate of glycerol

UNIT-VI: Protein Metabolism (6 Hours)
Transamination, Deamination and Ornithine Cycle.

PRACTICAL (30 Hours)

1. Qualitative and quantitative tests for carbohydrates
2. Estimation of reducing and non-reducing sugars
3. Qualitative and quantitative tests for amino acids
4. Separation of amino acids by paper chromatography
5. Qualitative and quantitative tests for lipids
6. Qualitative and quantitative tests for proteins
7. Estimation of proteins
8. Determination of pH and use of pH meter.
9. Study of metabolism through charts.

SUGGESTED READINGS:

1. Tortora, G.J. and Derrickson, B.H. (2009). Principles of Anatomy and Physiology, XII
Edition, John Wiley & Sons, Inc.

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