Page 58 - CatalogNEP-LS

ER, Golgi body: Structures and roles.
Peroxisomes, Glyoxisomes and vacuole: Structures, composition, functions in animals and plants
Nucleus: Nuclear E n v e l o p e - s t r u c t u r e o f n u c l e a r p o r e complex; chromatin;
molecular organization, DNA packaging in eukaryotes, euchromatin and heterochromatin,
nucleolus and ribosome structure (brief).
UNIT – III (15 Hours)

Cell Membrane and Cell Wall: The functions of membranes; Models of membrane structure; The
fluidity of membranes; Membrane proteins and their functions; Carbohydrates in the membrane;
Selective permeability of the membranes; Cell wall. Cell Cycle: Overview of Cell cycle, Mitosis
and Meiosis. Replication, transcription, and translation in prokaryotes; Regulation of gene
expression: Prokaryotes: Lac operon and Tryptophan operon

PRACTICAL (30 Hours)

1. To study prokaryotic cells (bacteria), viruses, eukaryotic cells with the help of light and
electron micrographs.
2. Study of the photomicrographs of cell organelles
3. To study the structure of animal cell through temporary mounts.
4. Study of mitosis and meiosis (temporary mounts and permanent slides).
5. Prepare the slide of bar body in human cheek cells.
6. Measure the cell size (either length or breadth diameter) by micrometry.
7. Study the structure of nuclear pore complex by photograph (from Gerald Karp) Study of
special chromosomes (polytene & lamp brush) either by slides or photographs.
8. Study DNA packaging by micrographs.
9. To study cell structure from onion leaf peels.
10. Demonstration of staining and mounting methods
11. Study of electron micrograph of viruses, bacteria, cyanobacteria and eukaryotic cells for
comparative cellular organization.

SUGGESTED READINGS:

1. Cell Biology and Molecular Biology by D. Robertis, EDP & DE Robertis E.M.F, 8th
ed., Saunders & Co. Philadelphia (1995).
2. Cell Biology by C.B. Powar, 3rd ed., Himalaya Pub., Bombay (1984).
3. Advances in Cell and Molecular Biology by Dupraw, E.J., eds. Academic Press, New
York & London (1971).
4. Cell Biology. Pollard, Thomas, D.S. Earnshan, William C. Saunders. USA. (2002).
5. The Cell: A Molecular Approach by Cooper GM & H 5th edition (2009).
6. Molecular Biology of the Cell, 5th edition by Alberts, John Raff. Roberts & Walter
(2008).
7. Cell and Molecular Biology: Concepts and Experiments 5th edition by Gerald Karp.
John Wiley and Saunders. 8. Molecular Cell Biology by Lodish et. al. W.H. Freeman
Co. New York (2008).

Zoo.222 Biochemistry 3+1

LEARNING OBJECTIVES:

The primary objective of this course is:

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