VPT-610    PHARMACOLOGICAL TECHNIQUES    (1+1)

OBJECTIVE

To impart the knowledge of various basic pharmacological techniques and screening methods of drugs.

THEORY

UNIT I

Principles of drug action and bioassay. Dose response curves and their analysis.

UNIT II

Techniques for setting up isolated and intact preparations.

UNIT III

Organization of screening programme of drugs; multidimensional screening procedures and gross observational methods.

PRACTICAL

Setting up of isolated and intact preparations, recording of BP in dog/rat, ecording of ECG in rat, experiments on drug potentiation, antagonism and achyphylaxis. Construction of dose-response plots, calculation of EC50, issociation rate constants, potency ratio, pAx, pDx and pD’x values.

Specific tests for evaluation of tranquillizing, hypnotic, analgesic, anticonvulsant, eneral and local anesthetic, muscle relaxant, antiinflammatory, ntipyretic, antiarrhythmic, antihypertensive, ntihyperglycemic and anticholesterimic activities. Determination of otency ratio, median effective, toxic or lethal doses. Bioassay techniques.

 

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