Page 155 - CatalogNEP-PS

Microorganisms involved in phosphorus solubilisation: bacteria and fungi - Isolation,
characterization, mass inoculum production, field application

UNIT 4 Mycorrhizal Biofertilizers (4 Hours)
Importance of mycorrhizal inoculum, types of mycorrhizae and associated plants, Mass inoculum
production of VAM, field applications of Ectomycorrhizae and VAM.

PRACTICAL (30 Hours)

1. To study the principle and applications of important instruments
used in the microbiology laboratory.
2. Preparation of culture media and sterilization for bacterial
cultivation.
2. Sterilization of glassware using Hot Air Oven and assessment for sterility.
3. Isolation of pure cultures of bacteria by streaking method.
4. Preservation of bacterial cultures by various techniques.
5. Estimation of CFU count by spread plate method/pour plate method.
6. Isolation of Rhizobium from leguminous plants.
7. Isolation of free living nitrogen fixing bacteria (Azotobacter) from soil.
8. Isolation of P-solubilizers from rhizospheric soil.
9. Staining of mycorrhizal fungi colonized roots.
10. Preparation of liquid and carrier based formulations of biofertilizers and their field
application.

SUGGESTED READINGS:

1. Mahendra K. Rai. Hand Book of Microbial Biofertilizers. The Haworth Press, Inc. New
York. 2005.
2. Shah Fahad, Shah Saud, Fazli Wahid, Muhammad Adnan. Biofertilizers for Sustainable
Soil Management. CRC Press. 2023.
3. Coyne MS. Soil Microbiology: An Exploratory Approach. Delmar Thomson Learning.
2001. Print
4. Reddy, S.M. et. al. Bioinoculants for Sustainable Agriculture and Forestry. Scientific
Publishers. 2002.
5. Subba Rao NS. Soil Microbiology. 4th edition. Oxford & IBH Publishing Co. New
Delhi, 1999.
6. K. R. Aneja. Experiments in Microbiology, Plant Pathology, Tissue Culture and Microbial
Biotechnology. New Age International Private Limited. 2022.

IAPC122/ Agron.361 Organic Farming 1+1

LEARNING OBJECTIVES:

The primary objective of this course is:
 To create awareness among the students about organic farming and its importance in
sustainable agriculture.
 To provide a skill set of Organic farming to students to help them become self-reliant.

LEARNING OUTCOMES:

This course will enable the students to understand:

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