Page 128 - CatalogNEP-PS

Ultra Violet and Visible Spectroscopy: Electronic transitions (185-800 nm), Beer- Lambert Law,
Effect of solvent on electronic transitions, Ultra Violet bands of carbonyl compounds, unsaturated
carbonyl compounds, dienes, conjugated polyenes, Steric effect in biphenyls, Fieser- Woodward
rules for conjugated dienes and carbonyl compounds, ultra violet spectra of aromatic and
heterocyclic compounds. Applications of UV- visible spectroscopy in organic chemistry.

UNIT -II (11 Hours)
Infrared Spectroscopy: Principle, Instrumentation and sample handling, Characteristic vibrational
frequencies of common organic compounds, Effect of hydrogen bonding and solvent effect on
vibrational frequencies, overtones, combination bands and Fermi resonance. Introduction to
Raman spectroscopy. Applications of IR and Raman spectroscopy in organic chemistry.

UNIT –III (12 Hours)
Nuclear Magnetic Resonance (NMR) Spectroscopy: General introduction, chemical shift, spin-
spin interaction, shielding mechanism, chemical shift values and correlation of protons present in
different groups in organic compounds. chemical exchange, effect of deuteration, complex spin-
spin interaction between two, three, four and five nuclei, virtual coupling. Stereochemistry,
hindered rotation, Karplus- relationship of coupling constant with dihedral angle. First and second
order spectra, Simplification of complex spectra-nuclear magnetic double resonance, spin tickling,
INDOR, contact shift reagents, solvent effects. Fourier transform technique, nuclear Overhauser
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effect (NOE). Introduction to resonance of other nuclei – C NMR, 2-D and 3-D NMR,
Applications of NMR in organic chemistry.

UNIT -IV (11 Hours)
Mass Spectrometry: Introduction, ion production—EI, CI, FD and FAB, factors affecting
fragmentation, ion analysis, and ion abundance. Mass spectral fragmentation of organic
compounds with common functional groups, Molecular ion peak, Meta-stable peak, McLafferty
rearrangement. Nitrogen Rule. Examples of mass spectral fragmentation of organic compounds
with respect to their structure determination. Introduction to negative ion Mass spectrometry,
TOF-MALDI.

*Tutorial (15 Hours)

The students will:
 Interpret and analyse spectra of the molecules using rules of various spectroscopic
techniques
 Solve problems by choosing suitable spectroscopic methods and interpreting
corresponding data
 Solve problems based upon IR, UV, NMR and mass spectroscopy.

SUGGUESTED READINGS:

1. Practical NMR Spectroscopy, M.L. Martin, J.J. Delpeuch and G.J. Martin, Heyden.
2. Spectrometric Identification of Organic Compounds, R.M. Silverstein, G.C. Bassler and
T.C. Morrill, John Wiley.
3. Introduction to NMR Spectroscopy, R.J. Abraham, J. Fisher and P. Loftus, Wiley.
4. Application of Spectroscopy of Organic Compounds, J.R. Dyer, Prentice Hall.
5. Spectroscopic Methods in Organic Chemistry by D.H. Williams,I. Fleming, Tata McGraw-
Hill.
6. Organic spectroscopy by Jagmohan
7. Organic spectroscopy by W. Kemp.
8. Spectroscopy by Pavia

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