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conventional methods of animal vaccine production, recombinant approaches to vaccine
production, modern vaccines.
UNIT – IV (6 Hours)

Overview of genomics – definition, complexity, and classification; need for genomics level
analysis; methods of analyzing genome at various levels – DNA, RNA, protein, metabolites and
phenotype; genome projects and bioinformatics resources for genome research – databases;
overview of forward and reverse genetics for assigning function for genes
RNAi, CRISPR-CAS; History of its discovery, elucidation of the mechanism including
introduction to all the molecular players, development of applications for in vivo genome
engineering for genetic studies, promise of the technology as a next generation therapeutic
method.

PRACTICALS (30 Hours)

1. Introduction to Instruments used in Molecular Biology /GE Laboratory
2. Preparation of Buffers.
3. DNA isolation and DNA quantitation
4. Polymerase Chain Reaction and analysis by agarose gel electrophoresis
5. Transformation of E.coli with standard plasmids and calculation of transformation efficiency
6. Confirmation of the insert by Colony PCR and Restriction mapping
7. Explore different softwares of bioinformatics analysis

SUGGESTED READINGS:

1. Old, R. W., Primrose, S. B., & Twyman, R. M. (2001). Principles of Gene Manipulation: An
Introduction to Genetic Engineering. Oxford: Blackwell Scientific Publications.
2. Green, M. R., & Sambrook, J. (2012). Molecular Cloning: a Laboratory Manual. Cold Spring
Harbor, NY: Cold Spring Harbor Laboratory Press.
3. Brown, T. A. (2006). Genomes (3rd ed.). New York: Garland Science Pub.
4. Glick, B. R., & Pasternak, J. J. (2010). Molecular Biotechnology: Principles and Applications
of Recombinant DNA. Washington, D.C.: ASM Press.
5. Umesha, S. (2013). Plant Biotechnology. The Energy and Resources.

RESEARCH/PROJECT/SEMINAR:

Zoo.391 Seminar 0+1

LEARNING OBJECTIVES:

The primary aim of this course is to:
 Identify and compare technical and practical issues related to the area of course
specialization.
 Outline annotated bibliography of research demonstrating scholarly skills.
 Prepare a well-organized report employing elements of technical writing and critical
thinking.
 Demonstrate the ability to describe, interpret and analyze technical issues and develop
competence in presenting.

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