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cell suspension culture, media preparation – nutrients and plant hormones; sterilization
techniques; applications of tissue culture - micropropagation; somaclonal variation; androgenesis
and its applications in genetics and plant breeding; germplasm conservation and cryopreservation;
synthetic seed production; protoplast culture and somatic hybridization - protoplast isolation;
culture and usage; somatic hybridization - methods and applications; cybrids and somatic cell
genetics; plant cell cultures for secondary metabolite production.Enzymes useful in molecular
cloning: Restriction endonuclease, DNA ligases

PRACTICAL (30 Hours)

1. Introduction to Instruments used in Biotechnology Laboratory
2. Preparation of Stock Solutions, buffers and pH determination
3. Sterilization techniques
4. Media Preparation and Aseptic Transfer Technique
5. Isolation and purification of DNA (genomic)
6. Agarose Gel Electrophoresis of the genomic DNA
7. Detection of DNA by gel electrophoresis
8. Estimation of DNA by UV spectroscopy

SUGGESTED READINGS:

1. Stryer, L. (2015). Biochemistry. (8th ed.) New York: Freeman.
2. Lehninger, A. L. (2012). Principles of Biochemistry (6th ed.). New York, NY: Worth.
3. Chawla, H. S. (2000). Introduction to Plant Biotechnology. Enfield, NH: Science.
4. An Introduction to Practical Biochemistry (2017) 3rd ed., Plummer, D.T., McGraw Hill
Education, ISBN: 978-0070994874.
5. Brown, T. A. (2006). Genomes (3rd ed.). New York: Garland Science Pub.

Bot.325/Zoo.325 Introduction to Bioinformatics 2+1

LEARNING OBJECTIVES:

 To acquaint students with the important concepts related to bioinformatics
 To understand the role of Bioinformatics in science and daily life activities
 To provide introductory exposure to various Bioinformatics databases and tools

LEARNING OUTCOMES:

At the end of this course, the student will be able to-
 Describe contents and properties of some important bioinformatics databases and tools.
 Apply and retrieve various data from Biological databases and perform basic DNA and
protein sequence analysis
 Perform text- and sequence-based searches,analyze and discuss the results in light of
molecular biological knowledge

THEORY (30 Hours)

UNIT – I: (10 Hours)

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